microplates or in a cuvette at a. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Plate Formats: 6 to 384-well microplates. Learn more 0200-7008 6- to 1536-well plates. #3610) BrightMax sealing films (Genesee cat. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. Description. You can add your own protocols. 96-well microplate compatible Well read area range: D4 to F11 384-well microplate compatible Well read area range: H7 to K22 Figure 1-3 24-Well Micro-VolumeLearn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. validation, maintenance, and repair documentation. 1 sec. Figure 2. Step 2: Launch SoftMax Pro Software. No accessories are needed and SoftMax® Pro Software. 96-well: 0. Detergent Compatible Bradford assay. 96- or 384-well microplate compatibility. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Fluorescence Microplate Reader Comparison Chart. Supplier: Molecular Devices. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. 384-well: 0. While they play an important role in generating data from a wide range of. (96, 384, or 1536 wells) and the "Select Specific". For biochemical. With various on-board features and. SpectraMax iD5. g. (FP) configured microplate readers. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Blank wells containing 50 µL culture medium only (n = 8) were also prepared. F3/F5 Multi-Mode Microplate Readers. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well3. 50 µL of. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Or scan up to 384 wells at any time to determine the best assay conditions. Published Dec 16, 2020 | Updated Dec 16, 2020. 747. Please refer to the Obsolete Microplate Reader Models for more information. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Seller assumes all responsibility for this listing. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Endpoint, kinetic, spectrum. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 020 fmol 0. 2022 Harini Natarajan et al. white 96 well plate and 75. #N207) LAL Reagent Water (Lonza cat. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Wavelength Range 200 to 999 nm. The color touch screen provides a visual user interface, making programming fast and intuitive. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. Our microplate. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Three low volume 24-well bottom slides. for ValitaTiter Plus standards read on Molecular Devices readers. 5°C at 37°C. Plate wash time (300 µl). The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Three kits covering a wide range of dsDNA concentrations. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 3 OD at 200 nm. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. Listing: #4772697. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. Dynamic range > 9 orders of magnitude. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. Open the catalog to page 5. Consult Molecular Devices's entire Threshold Immunoassay System catalogue on MedicalExpo. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). . Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. San Jose, California, June 5, 2018. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. 05. robotics-automation-compatible. 030 fmol Fluorescence Polarization (FP). Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. personalized as your research. the appropriate well of the microplate. 0 H x 60. Documentation:1. The OptiMax by Molecular Devices is a tunable microplate reader. 2600A October 2023. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. With various on-board features and capabilities, the ThermoMax is. Well scanning With well scanning, multiple points within each well can be read, providing a high. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Unparalleled performance on a personalized platform. Published Dec 16, 2020 | Updated Dec 16, 2020. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Growth medium was then replaced with fatty acid uptake solution. 9. 2. 48 W x 12. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. 500 USD. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. and luminescence microplate reader validation DATA SHEET. 1 pM 1536-well: 0. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Softmax Pro GxP Software. Compare specifications and request a quote from leading suppliers. Technical specifications. 2 OD at 200 nm. Get. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. 69: 85. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. , Sigma cat. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. This reader also makes use of a unique temperature-independent methodology for path length correction. For more great deals on Microplate readers by Molecular Devices, please browse our Molecular Devices Microplate readers page. 012 fmol 0. For more sample throughput, the system can be. We would like to show you a description here but the site won’t allow us. the iD5 reader’s filter system. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Fluorescent DNA quantitation. 125 pM * Specifications listed are for europium 96-well: 0. Simple, economical . The CellTiter-Glo assay from Promega uses luciferase. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. in space. Temperature Control: ambient from +4 to 45C. Other features and benefits include: Eliminate changing out filters. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Four wash/rinse bottles are included and configurable for both 96- and 384. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. $489. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. adjust the reader. It includes both shaking and incubation functions, and is compatible with 96- well microplates. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Pick the best speed, sensitivity, and price for your needs. The spectrophotometer has a wide wavelength range 190. 384 well polystyrene microplates have plate dimensions of 127. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. Microplates . System setup and. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. maximum-illumination-channels. Abstract and Figures. Equipped with scanning, shaking and kinetics modules. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. #W50-100) SpectraMax® ABS Plus Microplate Reader. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. The FilterMax F3 can read 96-well and 384-well plates. > Molecular Devices LLC > microplate reader. Deep well blocks available in 24, 96 and 384-well formatsFlexStation® 3 Multi-Mode Microplate Readers . HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. MycoAlert Reagent was added to each well and incubated at room temperature for 5. For more sample throughput, the system can well. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. 2 mm x 86 mm) Height:. Formats available are strip washers, full plate washers and combination washer-dispensers. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. Create custom fluorescence assays without having to purchase expensive filter sets. ValitaTiter Assays Setup Guide 2601A October 2023. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. 3 OD at 200 nm. The screw remains inside the instrument. 1536-well: 0. M5. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. #N207) LAL Reagent Water (Lonza cat. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. 375 pM. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. g. 3:40 . Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. automation for any lab. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. Microplate reader assays requiring infrared (IR) or red-shifted. 4. ValitaTiter 96-well (Pack of 20). BMG LABTECH. Infinite 200 Pro Microplate Reader. #G7294). 98 Bovine serum albumin mg/mL O. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Plates can put in the drawer both in landscape and portrait positions. Infinite 200 Pro Microplate Reader. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. 5. 5 mm and 15. 96-well: 0. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Microplate Readers. Place the microplate in the drawer. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. This is equivalent to having 511 built-in filters,Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. 340‚ 850 nm. 63. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Flexibility to read a variety of plate formats. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. Learn more. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. This unlocks the transport slide. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. Outer wells are aligned for proper viewing with FLIPR. #N207) LAL Reagent Water (Lonza cat. Please refer to the Obsolete Microplate Reader Models for more information. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. section, select plate section titled. #L6876) Guanidine hydrochloride (Sigma cat. A microplate reader as . 96-well microplate at 200 µL/well. No filter change with dual monochromator optics. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Category 2 and 3 microplate readers use gradient heating as well. Keywords: fluorescence polarization, trf, fret, igg. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Both readers were heated to 37ºC prior to setting up . 0 500 1000 1500 2000 2500 3000 3500 time (sec. No additional manuals are available. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 96-well solid bottom, clear polystyrene microplates (Greiner cat. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. 100 fmol 1536-well (8 µL) 0. The concentration of total free thiols in the protein. M5. The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. 2 mm x 86 mm) Height: between 13. The VersaMax and SpectraMax 190 read 96-well microplates. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. Compare this item. SpectraMax® Microplate Reader Suite of Products Knowledge Base. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Lucid Scientific. SoftMax Pro Software’s. It measures the optical density (OD) of samples in both 96- and 384-well. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. EMax Plus Microplate Reader, Molecular Devices,96 Well Plate, LLC (CAT#: STEM-LE-0510-LC) Cytation™ 1 Cell Imaging Multi-Mode Reader, Wavelength Range: 200 to 700 nm, BioTek Instruments (CAT#: STEM-MR-0003-LKN) Perkin Elmer EnVision 2105-0020 Multilabel Microplate Reader, Perkin Elmer (CAT#: STEM-S-0723-LGZ)The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. 7. Learn more about how absorbance is measured, and some key applications that utilize. Average StDev (mP) 1. Four wash/rinse bottles are included and configurable for both 96- and 384. CloneMatrix. Reading a Microplate . The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Monochromatic filter is equipped. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Use black microplates. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. book Page 1 Wednesday, October 12, 2011 4:56 PMBuilt around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene. 4. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. Detection modalities are shown in Table 1-1. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Condition: Used - Good. Reading Capabilities: cuvette or microplate. The eight-channel system delivers both high precision and speed when reading 96-well microplates for endpoint measurements, kinetic assays, and spectral scans. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. , Sigma cat. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Both readers were heated to 37ºC prior to setting up the assay. 6. microplates or in a cuvette at a selected. Instrument settings can be saved as a protocol file and used repeatedly for reading differentMolecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. flat meniscus), but is approx 15 mL. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. 0 500 1000 1500 2000 2500 3000 3500 time (sec. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. 2 2601A ValitaTiter. The system is operated through the industry-leading SoftMax® Pro. Temperature control. #1402-9700) 384-well low volume solid black microplate (Corning cat. 7°C (gradient heating design). CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. • 0. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Integrated stacker option. 03 pM 384-well: 0. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Step 3: Click “Settings” and a screen will appear (Figure 1). Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. 2 Pages. Triplicates of each BSA concentration were made for this assay plate. 300 µL of Detergent Compatible Bradford reagent was added to each well. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates.